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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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Democratization of Copper Analysis in Grape Must Following a Polymer-Based Lab-on-a-Chip Approach.

José Carlos Guirado-Moreno1, Israel Carreira-Barral1, Saturnino Ibeas1

  • 1Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain.

ACS Applied Materials & Interfaces
|March 20, 2023
PubMed
Summary

A new, low-cost smartphone method accurately measures copper in grape must, simplifying wine production quality control. This rapid, in situ analysis offers a viable alternative to traditional lab testing for wineries.

Keywords:
RGB parameterscolorimetrycopper detectiongrape mustsensory polymerswine industry

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Sensor Technology

Background:

  • Food industry quality control is critical for safety, sensory attributes, and commerce.
  • Accurate copper determination in grape must is essential for wineries during wine production.
  • Traditional methods like flame atomic absorption spectrometry (FAAS) and inductively coupled plasma mass spectrometry (ICP-MS) require laboratory analysis.

Purpose of the Study:

  • To develop a straightforward, rapid, and inexpensive analytical tool for in situ copper content determination in grape must.
  • To create a user-friendly method suitable for wineries, even with unskilled personnel.
  • To validate a novel colorimetric polymer sensor combined with smartphone technology.

Main Methods:

  • Development of a film-shaped colorimetric polymer sensor that changes color in response to copper ions.
  • Utilizing smartphone imaging and a dedicated app for automatic color analysis of the sensor.
  • Comparison of the proposed method with traditional FAAS for copper content analysis in real wine production samples.

Main Results:

  • The proposed method demonstrated a limit of detection for copper of 0.08 ppm.
  • Analysis of 18 production grape must samples showed copper content ranging from 0.41 to 6.08 ppm.
  • Statistical analysis confirmed full consistency between the smartphone-based method and FAAS, validating the new approach.

Conclusions:

  • The developed colorimetric polymer sensor and smartphone application provide a valid, rapid, and cost-effective method for copper determination in grape must.
  • This innovative approach supports quality control in wineries, enabling in situ analysis without specialized laboratory equipment.
  • The technology offers a practical solution for wineries to monitor copper levels throughout the wine production process.