Non-separation radioassay for cortisol binding capacity in human serum

A radioassay for the measurement of cortisol binding capacity in human serum was developed and the results validated by radioimmunoassay of serum corticosteroid binding globulin. The mini-scale method is simple, reliable and rapid, and avoids separation of partitioned phases. The linear correlation between the free cortisol index and free cortisol concentration as calculated by the two methods utilizing serum samples with normal and abnormal protein concentrations was r = 0.962 (n = 114, p less than 0.001) and the regression equation was y = 3.516 x - 2.632. The technique requires no preparation of reagents, and the assay can be performed in 45 minutes using 0.05 ml of serum or plasma.