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Direct kinetic method for the determination of phosphate
1Department of Clinical Chemistry, Municipal Hospital Slotervaart, Amsterdam, The Netherlands.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|August 1, 1987
Summary
Accurate and economical serum phosphate measurement is achieved using a spectrophotometric method. This optimized procedure, suitable for discrete analyzers, addresses potential interferences in patient samples.
Area of Science:
- Clinical Chemistry
- Analytical Biochemistry
Background:
- Accurate serum phosphate measurement is crucial for diagnosing various metabolic disorders.
- Existing methods may face limitations in precision, cost, or applicability to automated analyzers.
Purpose of the Study:
- To develop and optimize an initial rate spectrophotometric procedure for serum phosphate determination.
- To validate the method's performance on a discrete analyzer and assess potential interferences.
Main Methods:
- Utilized an initial rate spectrophotometric assay measuring phosphomolybdate complex formation at 334 nm.
- Optimized polyvinylpyrrolidone concentration for analysis on the Eppendorf Epos discrete analyzer.
Main Results:
- The developed spectrophotometric procedure provides accurate and precise serum phosphate measurements.
- The method is economical and suitable for routine clinical laboratory use.
- Interference from hyperlipaemia, high bilirubin, and high protein was evaluated.
Conclusions:
- The optimized initial rate spectrophotometric method offers a reliable and cost-effective approach for serum inorganic phosphate analysis.
- This method is well-suited for implementation on discrete automated analyzers.
- Understanding and mitigating potential interferences are key for accurate clinical interpretation.