Related Experiment Video

Updated: Aug 6, 2025

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator
10:48

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

Published on: December 28, 2017

9.6K

VEGF affects mitochondrial ROS generation in glioma cells and acts as a radioresistance factor

Genro Kashino1, Shinko Kobashigawa2, Aoki Uchikoshi2

  • 1Radioisotope Research Center, Nara Medical University, Shijo-Machi, Kashihara, Japan. kashino@naramed-u.ac.jp.

Radiation and Environmental Biophysics
|March 21, 2023
PubMed

Insights

Vascular endothelial growth factor (VEGF) protects cells from radiation by reducing oxidative stress and altering mitochondrial function. This effect on cell survival is independent of DNA double-strand break induction.

Area of Science:

  • Cellular biology
  • Radiation oncology
  • Molecular medicine

Background:

  • Vascular endothelial growth factor (VEGF) is crucial for angiogenesis and a target in anticancer therapies.
  • The role of VEGF in cellular response to ionizing radiation, particularly concerning cell-cell communication and radiosensitivity, remains unclear.

Purpose of the Study:

  • To investigate the influence of VEGF on the radiosensitivity of cells.
  • To elucidate the underlying mechanisms of VEGF's effect on cellular response to ionizing radiation.

Main Methods:

  • Cultured rat C6 glioma cells were treated with recombinant VEGF (rVEGF) or endogenous VEGF.
  • Cells were subjected to X-ray irradiation.
  • Radiosensitivity, oxidative stress levels, DNA double-strand breaks (DSB), and mitochondrial oxygen consumption rate (OCR) were assessed.

Main Results:

  • Recombinant and endogenous VEGF exhibited radioprotective effects on C6 glioma cells against X-ray irradiation.
  • VEGF treatment reduced oxidative stress and decreased mitochondrial oxygen consumption rate (OCR).
  • VEGF did not affect the number of DNA double-strand breaks (DSB) immediately after irradiation.

Conclusions:

  • VEGF enhances cell survival after irradiation by modulating oxidative conditions via mitochondrial function.
  • The radioprotective mechanism of VEGF is independent of its effect on the induction of DNA double-strand breaks.
Keywords:
MitochondriaROSRadioprotective effectVEGF

More Related Videos

Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care12:55

Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

Published on: February 16, 2015

21.5K
Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts07:52

Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts

Published on: April 26, 2018

9.0K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.6K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
14.7K