Labeling approaches for DNA-PAINT super-resolution imaging

Area of Science:

• Molecular Biology
• Microscopy
• Biotechnology

Background:

• Super-resolution imaging techniques are crucial for visualizing biological structures at high resolution.
• DNA-PAINT (Direct DNA Labeled-based REtrieval And Nanostructure Probing) is a single-molecule localization microscopy method.
• DNA-PAINT provides ultra-high resolution and multiplexing capabilities for advanced biological imaging.

Purpose of the Study:

• To review the importance of DNA nanostructures in showcasing DNA-PAINT capabilities.
• To discuss the design of imager-docking strand pairs for DNA-PAINT imaging.
• To summarize labeling probes and chemical modification strategies for DNA-PAINT in biological samples.

Main Methods:

• Discussion of patterned DNA nanostructures for demonstrating DNA-PAINT.
• Analysis of imager-docking strand pair designs for imaging applications.
• Review of labeling probes and chemical conjugation strategies for docking strands.

Main Results:

• DNA nanostructures are vital for illustrating DNA-PAINT's potential.
• Various imager-docking strand combinations enable diverse imaging scenarios.
• A range of labeling probes have been developed to enhance resolution and multiplexing.

Conclusions:

• Effective implementation of DNA-PAINT in biological systems relies on docking strand-conjugated binders.
• Researchers have developed diverse labeling probes for improved resolution and multiplexing in DNA-PAINT.
• This review consolidates strategies for chemically modifying probes with docking strands for cellular DNA-PAINT.