Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hemoglobin01:24

Hemoglobin

3.9K
Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well.
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
3.9K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

10.8K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
10.8K
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

564
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
564
Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

459
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
459
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

1.4K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same author

Crystallise, poise, capture: a multimodal platform for correlated structural and spectroscopic characterisation of redox enzymes.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026
Same author

Integrated structural dynamics uncover a new B<sub>12</sub> photoreceptor activation mode.

Nature·2026
Same author

Increasing X-ray energy improves data quality in serial crystallography.

Journal of synchrotron radiation·2026
Same author

Time-resolved serial synchrotron and serial femtosecond crystallography of heme proteins using photocaged nitric oxide.

IUCrJ·2025
Same author

Sea foam contains hemoglycin from cosmic dust.

RSC advances·2024
Same journal

Anharmonic phonons via quantum thermal bath simulations.

The Journal of chemical physics·2026
Same journal

Quantum simulation of alignment dependent differential cross sections in co-propagating molecular beams at cold collision energies.

The Journal of chemical physics·2026
Same journal

Non-additive ion effects on the coil-globule equilibrium of a generic polymer in aqueous salt solutions.

The Journal of chemical physics·2026
Same journal

Insights into the unexpected small reduction of the temperature of maximum density of water by lithium chloride addition.

The Journal of chemical physics·2026
Same journal

Optical frequency comb double-resonance spectroscopy of the 9030-9175 cm-1 states of ethylene.

The Journal of chemical physics·2026
Same journal

Time reversal breaking of colloidal particles in cells.

The Journal of chemical physics·2026
See all related articles

Related Experiment Video

Updated: Aug 6, 2025

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
05:18

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

9.8K

Hemoglycin visible fluorescence induced by x rays.

M W McGeoch1, R L Owen2, S Jaho2

  • 1PLEX Corporation, 275 Martine St., Suite 100, Fall River, Massachusetts 02723, USA.

The Journal of Chemical Physics
|March 22, 2023
PubMed
Summary
This summary is machine-generated.

Hemoglycin, an iron-glycine polymer found in meteorites, exhibits unique light absorption and re-emission properties. Researchers observed visible light emission from hemoglycin crystals when exposed to X-ray irradiation.

More Related Videos

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

1.4K
Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
15:27

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

17.0K

Related Experiment Videos

Last Updated: Aug 6, 2025

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
05:18

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

9.8K
Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

1.4K
Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
15:27

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

17.0K

Area of Science:

  • Astrochemistry
  • Materials Science
  • Spectroscopy

Background:

  • Hemoglycin, a polymer of iron and glycine, has been identified in carbonaceous meteorites.
  • Iron atoms cap a glycine beta sheet, imparting unique visible and near-infrared absorptions absent in glycine alone.

Purpose of the Study:

  • To investigate the optical properties of hemoglycin, specifically its light re-emission under X-ray irradiation.
  • To characterize the emission spectra of hemoglycin crystals.

Main Methods:

  • Theoretical prediction and experimental observation of hemoglycin's 483 nm absorption.
  • X-ray irradiation of a hemoglycin crystal.
  • Measurement of visible light re-emission spectra.

Main Results:

  • Visible light re-emission was observed during X-ray irradiation of a hemoglycin crystal.
  • The emission spectrum was dominated by bands centered at 489 nm and 551 nm.
  • These findings confirm theoretical predictions and experimental observations of hemoglycin's optical behavior.

Conclusions:

  • Hemoglycin exhibits distinct photophysical properties, including visible light re-emission upon X-ray excitation.
  • The study provides insights into the interaction of X-rays with extraterrestrial organic molecules.
  • Further research may explore hemoglycin's role in astrobiology and its potential applications.