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A rapid and sensitive method for the identification and quantitation of sub-picomolar amounts of neurohypophysial
1Department of Dentistry, University of Adelaide, South Australia.
Life Sciences
|December 28, 1987
Abstract:
A simple, rapid and sensitive method, using isocratic reversed-phase HPLC, is described for the concomitant identification and quantitation of low levels of the various neurohypophysial peptides in biological tissues and fluids. The method requires little or no sample preparation and utilises UV peak detection at 215 nm with a serial signal amplification system to achieve a usable maximum sensitivity of less than 200 fmol of peptide.