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Clinical and Experimental Study of High Mobility Group Box-2 and Valvular Calcification in Elderly Patients with

Hongtao Huang1, Qingpiao Sun2, Yu Huang3

  • 1Nantong University Medical School, Nantong, China, 2013320091@stmail.ntu.edu.cn.

Cardiology
|March 23, 2023
PubMed

Insights

High mobility group box-2 (HMGB2) is linked to valve calcification in senile degenerative heart valve disease. HMGB2 may promote calcification by activating the NF-κB pathway and increasing BMP-4 expression.

Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • Biochemistry

Background:

  • Senile degenerative heart valve disease (SDHVD) is a growing clinical concern.
  • Valve calcification is a key pathological feature of SDHVD, leading to significant morbidity.
  • The molecular mechanisms underlying SDHVD valve calcification require further elucidation.

Purpose of the Study:

  • To investigate the association between high mobility group box-2 (HMGB2) and valve calcification in SDHVD.
  • To explore the potential role of HMGB2 in the pathogenesis of SDHVD valve calcification.

Main Methods:

  • Serum HMGB2 levels were measured in patients with and without calcified heart valves using ELISA.
  • Human heart valve interstitial cells (hVICs) were cultured in vitro and subjected to calcification induction.
  • Protein and gene expression of HMGB2, BMP-4, and NF-κB P65 were analyzed using Western blot and RT-PCR.

Main Results:

  • Serum HMGB2 levels were significantly higher in patients with calcified heart valves compared to controls.
  • In vitro, HMGB2 protein and BMP-4 expression were elevated in the calcification group of hVICs.
  • Exogenous HMGB2 administration upregulated BMP-4 and NF-κB P65 expression in hVICs.

Conclusions:

  • HMGB2 is correlated with valvular calcification in SDHVD.
  • HMGB2 may promote SDHVD valve calcification by activating the NF-κB pathway.
  • HMGB2's upregulation of BMP-4 suggests a key role in the calcification process.
Abstract

Keywords:
BMP-4HMGB2NF-κBSenile degenerative heart valve diseaseValve calcification

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