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Updated: Jul 11, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 18, 2010
Kinetic description of proteolysis. Part 4. Hydrolysis kinetics of partial protein hydrolysates
M M Vorob'ev1, L S Slobodyanikova, S V Vitt
1A. N. Nesmeyanov Institute of Organo-Element Compounds, USSR Academy of Science, Moscow.
Abstract:
This report presents experimental data on kinetics of casein hydrolysate hydrolysis. The experiments were interpreted in the framework of a theory reported, which is guided by an experimentally measurable value--a total molar concentration of all peptides and amino acids (total amino nitrogen). The total amino nitrogen N was determined by photometry of N-trinitrophenyl (N-TNP) derivatives obtained by means of trinitrobenzenesulfonic acid (TNBS). Unlike the procedures reported previously, we stopped the proteolysis reaction by boiling, and performed the trinitrophenylation in a 20% V. aqueous solution of MeCN.
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