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Gene expression in organoids: an expanding horizon
Artem Smirnov1, Gerry Melino1, Eleonora Candi2,3
1Department of Experimental Medicine, Torvergata Oncoscience Research, University of Rome "Tor Vergata", Via Montpellier 1, 00133, Rome, Italy.
Biology Direct
|March 25, 2023
Summary
Human organoid cultures offer new ways to study development and cancer. Advanced multi-omics technologies like single-cell transcriptomics are crucial for understanding gene expression in these complex models for precision medicine.
Area of Science:
- Biotechnology
- Developmental Biology
- Genomics
Background:
- Human three-dimensional organoid cultures are revolutionizing in vitro modeling for development and personalized cancer therapies.
- Organoid complexity necessitates advanced techniques to analyze global gene expression and molecular mechanisms.
- Organoid models offer new insights into human development and disease.
Purpose of the Study:
- To review state-of-the-art multi-omics technologies for studying organoids.
- To highlight the application of these technologies in understanding development and advancing precision medicine.
Main Methods:
- Single-cell transcriptomics
- Chromatin accessibility assays
- Multi-omics profiling
Main Results:
- Multi-omics technologies are being adapted for diverse human organoid tissues.
- These techniques enable the tracing of global gene expression changes.
- Identification of key players and molecular mechanisms in organoid development and disease.
Conclusions:
- Organoid cultures combined with multi-omics provide powerful tools for research.
- These integrated approaches are essential for modeling development and enabling precision medicine.
- Future applications include personalized cancer therapies and deeper understanding of human biology.
