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Related Concept Videos

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The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
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Related Experiment Video

Updated: Aug 5, 2025

Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo Organogenesis
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Using mammary organoids to study cilia.

Aurore M M Dupuy1, Philippe P Juin1, Vincent J Guen1

  • 1Nantes Université, Inserm, CNRS, Université d'Angers, CRCI(2)NA, Nantes, France.

Methods in Cell Biology
|March 26, 2023
PubMed
Summary
This summary is machine-generated.

This study details a method for culturing mouse mammary organoids and staining primary cilia. This technique aids in understanding ciliogenesis and ciliary signaling in tissue development.

Keywords:
CiliaCiliogenesisImmunofluorescenceOrganoidsSignaling

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Cilia are vital cellular structures involved in signaling and fluid movement.
  • Cilia dysfunction causes diseases known as ciliopathies.
  • Understanding ciliogenesis is crucial for tissue homeostasis and development.

Purpose of the Study:

  • To provide a protocol for generating mouse mammary organoids.
  • To establish a method for immunofluorescence staining of primary cilia within these organoids.
  • To facilitate research into ciliogenesis, ciliary signaling, and ciliary function.

Main Methods:

  • Isolation and culture of mouse mammary stem cells.
  • Three-dimensional culture to form mammary organoids.
  • Immunofluorescence staining protocol optimized for primary cilia detection in organoids.

Main Results:

  • Successful generation of mouse mammary organoids from stem cells.
  • Effective visualization of primary cilia within the organoid structures using immunofluorescence.
  • Demonstration of a robust protocol applicable to studying cilia in complex 3D models.

Conclusions:

  • Mammary organoids are a suitable model for studying primary cilia.
  • The presented protocol enables detailed investigation of ciliogenesis and ciliary function.
  • This research contributes to understanding cilia-related mechanisms in tissue development and disease.