A paired comparison of nerve dimensions using B-Mode ultrasound and shear wave elastography during regional...

Shear wave elastography (SWE) presents nerves in colour, but the dimensions of its colour maps have not been validated with paired B-Mode nerve images. Our primary objective was to define the bias and limits of agreement of SWE with B-Mode nerve diameter. Our secondary objectives were to compare nerve area and shape, and provide a clinical standard for future application of new colour imaging technologies such as artificial intelligence.

Eleven combined ultrasound-guided regional nerve blocks were conducted using a dual-mode transducer. Two raters outlined nerve margins on 110 paired B-Mode and SWE images every second for 20 s before and during injection. Bias and limits of agreement were plotted on Bland-Altman plots. We hypothesized that the bias of nerve diameter would be <2.5% and that the percent limits of agreement would lie ±0.67% (2 SD) of the bias.

There was no difference in the bias (95% confidence interval (CI) limits of agreement) of nerve diameter measurement, 0.01 (-0.14 to 0.16) cm, P = 0.85, equivalent to a 1.4% (-56.6% to 59.5) % difference. The bias and limits of agreement were 0.03 (-0.08 to 0.15) cm², P = 0.54 for cross-sectional nerve area; and 0.02 (-0.03 to 0.07), P = 0.45 for shape. Reliability (ICC) between raters was 0.96 (0.94-0.98) for B-Mode nerve area and 0.91 (0.83-0.95) for SWE nerve area.

Nerve diameter measurement from B-Mode and SWE images fell within a priori measures of bias and limits of agreement.