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Terminating substrates of DNA polymerases: synthesis and functional study
1Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Nucleic Acids Symposium Series
|January 1, 1987
Abstract:
In order to study the mechanisms of DNA biosynthesis a number of modified nucleoside - substrates of DNA polymerases was synthesized. The absence of hydroxyl at 3'-position of ribose results in terminating properties of DNA biosynthesis of these analogues. A single step synthesis of triphosphates and alpha-thiotriphosphates of natural and 3'-modified 2'-deoxynucleosides is described.