Chemoenzymatic synthesis of genes encoding medium-sized polypeptides by use of only one synthetic oligonucleotide

A novel strategy for the synthesis of genes encoding medium sized polypeptides from only one synthetic oligodeoxynucleotide is outlined. A 140-mer oligodeoxynucleotide forming a hairpin structure at its 3'-end has been synthesized and successfully used in the construction and cloning of a gene coding for salmon calcitonin-gly(33). Employing this "one oligonucleotide - one gene" approach the manual work required for oligodeoxynucleotide synthesis is reduced to a minimum.