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Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
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Updated: Aug 5, 2025

Fractionation of Lignocellulosic Biomass using the OrganoCat Process
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Mild Fractionation for More Sustainable Food Ingredients.

A Lie-Piang1, J Yang2, M A I Schutyser1

  • 1Laboratory of Food Process Engineering, Wageningen University, Wageningen, The Netherlands;

Annual Review of Food Science and Technology
|March 27, 2023
PubMed
Summary
This summary is machine-generated.

Resource-efficient food ingredient production is key to sustainability. Milder processing methods reduce environmental impact and enhance ingredient functionality, despite challenges with antinutritional factors and off-flavors.

Keywords:
dry fractionationfunctionalitymild wet separationplant proteinresource use efficiency

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Area of Science:

  • Food Science and Technology
  • Sustainable Processing
  • Ingredient Functionality

Background:

  • Increasing global challenges of food shortages, energy costs, and raw material scarcity necessitate a reduction in the food industry's environmental footprint.
  • Traditional extensive wet processing methods for food ingredients, while achieving high purity, contribute significantly to environmental impact due to energy-intensive steps like heating and dehydration.

Purpose of the Study:

  • To provide an overview of resource-efficient processes for food ingredient production.
  • To describe the environmental impact and functional properties associated with different processing methods.
  • To highlight the benefits of milder refining techniques for sustainability and ingredient quality.

Main Methods:

  • Comparison of extensive wet processing with milder wet alternatives (e.g., salt precipitation, water-only fractionation) and dry fractionation techniques (air classification, electrostatic separation).
  • Evaluation of environmental impact, focusing on energy consumption and resource use.
  • Assessment of functional properties of ingredients obtained through various methods.

Main Results:

  • Extensive wet processing has the highest environmental impact, primarily from heating and dehydration.
  • Milder processing methods, including dry fractionation and alternative wet methods, significantly reduce environmental impact by omitting or altering energy-intensive steps.
  • Milder refining processes enhance the functional properties of food ingredients, shifting focus from purity to desired functionality.

Conclusions:

  • Fractionation and formulation strategies should prioritize desired functionality over absolute purity to leverage benefits of milder processing.
  • Milder refining significantly reduces environmental impact, aligning with the growing trend towards sustainable food production.
  • Challenges such as antinutritional factors and off-flavors in mildly processed ingredients require further research and mitigation strategies.