Sugar Detection in Mosquitoes via Anthrone Tests

Adult mosquitoes feed on plant sugar, frequently in the form of floral nectar. However, because of spatial and temporal variation in this behavior, as well as to the predilection of most mosquitoes to alter their behavior in the presence of a researcher, observing mosquito nectar feeding and similar behaviors directly in real time is not always feasible. In this protocol, I describe methods for hot and cold anthrone tests, which can be used to quantify the degree to which mosquito sugar feeding occurs in nature.