Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

31.4K
Overview
31.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptional divergence of the zebrafish <i>sox17</i> lineage begins during gastrulation.

bioRxiv : the preprint server for biology·2026
Same author

In situ mutational screening and CRISPR interference define <i>apterous</i> cis-regulatory inputs during compartment boundary formation.

eLife·2026
Same author

Morphometric analyses of shape: The analysis software toolbox for quantification of craniofacial shape.

bioRxiv : the preprint server for biology·2025
Same author

Discovery of a pre-vein progenitor that requires VEGF/ERK inhibition to complete vein differentiation.

bioRxiv : the preprint server for biology·2025
Same author

Building a framework for reproducibility: the case for standardized data reporting and metadata integration in zebrafish research.

Disease models & mechanisms·2025
Same author

Elevated DNA damage without signs of aging in the short-sleeping Mexican cavefish.

eLife·2025

Related Experiment Video

Updated: Aug 5, 2025

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
10:00

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

3.2K

Next-generation plasmids for transgenesis in zebrafish and beyond.

Cassie L Kemmler1, Hannah R Moran1, Brooke F Murray2

  • 1University of Colorado, School of Medicine, Anschutz Medical Campus, Department of Pediatrics, Section of Developmental Biology, 12801 E 17th Avenue, Aurora, CO 80045, USA.

Development (Cambridge, England)
|March 28, 2023
PubMed
Summary

Researchers developed new genetic tools for zebrafish and other species. These advanced transgenesis reagents enhance gene expression studies and cell labeling for broader research applications.

Keywords:
CavefishEnhancer discoveryFlurophoresTrangenesis markerTransgenesisZebrafish

More Related Videos

Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

11.7K
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
10:22

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

Published on: June 25, 2015

10.8K

Related Experiment Videos

Last Updated: Aug 5, 2025

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
10:00

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

3.2K
Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

11.7K
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
10:22

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

Published on: June 25, 2015

10.8K

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Transgenesis is crucial for genetic modeling.
  • Tol2-based transgenesis and Gateway vectors have advanced zebrafish research.
  • There is a need for enhanced transgenic tools for broader applications.

Purpose of the Study:

  • To establish next-generation transgenesis tools for zebrafish and other species.
  • To expand and enhance transgenic applications in model organisms.
  • To provide versatile reagents for gene regulatory element testing and cell labeling.

Main Methods:

  • Generated Gateway middle entry vectors with promoters, fluorophores, CreERT2, and Gal4.
  • Developed new fluorescent proteins (mCerulean, mApple) and p2A peptide-based 3' vectors.
  • Created Tol2 destination vectors with zebrafish exorh promoter for pineal gland-specific expression.

Main Results:

  • New vectors facilitate gene regulatory element testing.
  • Extended color spectrum with novel fluorescent proteins and co-labeling strategies.
  • Established pineal gland-specific transgenesis markers active in zebrafish and cavefish.

Conclusions:

  • The developed vectors offer versatile reagents for transgenesis in zebrafish, cavefish, and other models.
  • These tools enhance gene expression studies, cell labeling, and tissue-specific marking.
  • The next-generation tools expand the scope of transgenic applications in model organisms.