Scanning electron microscopy of adenomatoid tumours

Area of Science:

• Urology
• Pathology
• Electron Microscopy

Background:

• Adenomatoid tumors are benign neoplasms of the epididymis.
• Their histogenesis remains a subject of debate, with the mesothelial theory being prominent.

Purpose of the Study:

• To investigate the ultrastructural morphology of adenomatoid tumors of the epididymis.
• To evaluate the utility of scanning electron microscopy (SEM) in examining these neoplasms.

Main Methods:

• Four adenomatoid tumors of the epididymis were processed using paraffin embedding and sectioning.
• Sectioned surfaces were examined using scanning electron microscopy in secondary electron mode.

Main Results:

• While solid tumor faces showed preparation-induced damage, the luminal surfaces of tubules were well-preserved.
• SEM revealed microvilli and a complex pattern of intersecting cord-like elevations on the luminal surfaces.
• Desquamating cells and thin cytoplasmic partitions (bridges) were observed within tubules.

Conclusions:

• The observed ultrastructural features support the mesothelial origin of adenomatoid tumors.
• Scanning electron microscopy, even with basic preparation, provides valuable insights into the internal structure of neoplasms.