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Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet
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Inactivation of SARS-CoV-2 on Surfaces by Cold-Plasma-Generated Reactive Species.

Som V Thomas1, Krista Dienger-Stambaugh2, Michael Jordan2

  • 1Department of Biomedical Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA.

Bioengineering (Basel, Switzerland)
|March 29, 2023
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Cold atmospheric plasma (CAP) effectively inactivates SARS-CoV-2. This study characterized reactive species and demonstrated significant viral load reduction using CAP treatment under ambient conditions.

Keywords:
COVID-19cold atmospheric plasmaoxygen reactive speciesvirus inactivation

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Area of Science:

  • Plasma physics
  • Virology
  • Biomedical engineering

Background:

  • SARS-CoV-2 poses a significant global health threat.
  • Effective and safe inactivation methods are crucial for infection control.
  • Cold atmospheric plasma (CAP) is an emerging technology with potential antimicrobial applications.

Purpose of the Study:

  • To design and develop a CAP apparatus for SARS-CoV-2 inactivation.
  • To characterize the reactive species generated by the CAP system.
  • To evaluate the efficacy of CAP treatment in reducing SARS-CoV-2 infectivity.

Main Methods:

  • A CAP apparatus was designed and built.
  • Optical Emission Spectroscopy (OES) was used to characterize reactive species (ROS and RNS).
  • Pseudotyped viral infectivity assays were performed to assess viral inactivation efficacy.

Main Results:

  • Various reactive oxygen species (ROS) and reactive nitrogen species (RNS) were identified.
  • CAP treatment for 8 seconds significantly reduced SARS-CoV-2 infectivity.
  • Increased exposure times (15-120 seconds) further enhanced viral inactivation.
  • Killing efficacy correlated with the type, intensity, energy, and frequency of reactive species.

Conclusions:

  • The developed CAP system effectively inactivates SARS-CoV-2.
  • ROS and RNS generated by CAP are responsible for viral inactivation.
  • CAP offers a promising ambient-condition method for controlling SARS-CoV-2 spread.