Development of Cellular Signaling Pathways by Bioceramic Heat Treatment (Sintering) in Osteoblast Cells
Yoona Jung1, Jooseong Kim2,3, Sukyoung Kim2
1Department of Physiology, Konkuk University School of Medicine, Chungju 27478, Republic of Korea.
Biomedicines
|March 29, 2023
Summary
Sintering bioceramics like hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP) impacts osteoblast cell signaling pathways. Sintered bioceramics altered calcium levels and modulated key pathways, suggesting effects on their clinical applications.
Area of Science:
- Biomaterials Science
- Cell Biology
- Materials Engineering
Background:
- Bioceramics, primarily calcium-phosphate materials, are crucial for bone tissue regeneration in medical and dental implants.
- The influence of bioceramic sintering on intracellular signaling pathways in osteoblasts is not well understood.
Purpose of the Study:
- To investigate the effects of sintering on the intracellular signaling pathways of osteoblast cells exposed to hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP).
Main Methods:
- Osteoblast cells were treated with sintered and non-sintered HA and β-TCP.
- X-ray diffraction was used to analyze material morphology.
- Intracellular signaling pathway proteins (JNK, Src, EGFR, p38, AKT) and their phosphorylated forms were quantified.
Main Results:
- Sintering altered HA morphology and increased calcium concentrations in both sintered HA and β-TCP.
- Both bioceramics inhibited the JNK pathway; sintered β-TCP showed minimal p-JNK expression.
- Sintering decreased p-Src levels, regulated p-EGFR by non-sintered forms, and reduced p-p38.
- All bioceramics attenuated total AKT, with non-sintered HA showing the most significant reduction; AKT phosphorylation was reduced except for non-sintered β-TCP.
Conclusions:
- Bioceramic sintering significantly affects multiple intracellular signaling pathways in osteoblasts.
- These findings provide insights into bioceramic functionality and may broaden their use as substrates in clinical settings.
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