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TEER and Ion Selective Transwell-Integrated Sensors System for Caco-2 Cell Model.

Elisa Sciurti1, Laura Blasi1, Carmela Tania Prontera1

  • 1National Research Council of Italy, Institute for Microelectronics and Microsystems, 73100 Lecce, Italy.

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|March 29, 2023
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Summary

New flexible sensors enable real-time monitoring of zinc and copper ions in cell cultures. This innovation aids in understanding ion transport and gut barrier integrity for improved gut-on-chip applications.

Keywords:
Caco-2 monolayerTranswell™ plateanodic stripping voltammetryelectrochemical impedance spectroscopy (EIS)organ-on-a-chip (OoC)transepithelial electrical resistance (TEER)

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cell Biology

Background:

  • Real-time ion monitoring in cell cultures is crucial for understanding biological processes and diseases.
  • Existing methods often lack the sensitivity or in-situ capabilities required for complex biological systems.

Purpose of the Study:

  • To develop and integrate novel flexible ion-selective sensors into cell culture systems for real-time ion transport analysis.
  • To assess cellular barrier integrity using electrochemical impedance spectroscopy (EIS).
  • To validate sensor performance and utility in a human intestinal Caco-2 cell model.

Main Methods:

  • Fabrication of flexible sensors coupling square wave anodic stripping voltammetry (SWASV) with ion-selective membranes (ISMs).
  • Integration of sensors into Transwell™ cell culture systems for zinc and copper ion monitoring.
  • In-situ electrochemical impedance spectroscopy (EIS) for cellular barrier integrity assessment.
  • Validation using transepithelial electrical resistance (TEER) and immunofluorescence.

Main Results:

  • Developed ion-selective sensors with high sensitivity (1 × 10-11 M) and low detection limits.
  • Successfully monitored zinc and copper ion transport across a Caco-2 cell monolayer.
  • EIS provided electrical parameters for effective cellular barrier integrity monitoring.
  • EGTA treatment confirmed as a permeability enhancer, validated by TEER and imaging.

Conclusions:

  • The developed flexible sensors are effective tools for real-time ion monitoring in cell culture and organ-on-a-chip systems.
  • The integrated approach allows simultaneous assessment of ion transport and cellular barrier function.
  • These sensors offer a prospective platform for studying gut metabolism, permeability, and related pathologies.