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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
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Rotaviruses in Wild Ungulates from Germany, 2019-2022
Nadine Althof1, Eva Trojnar1, Reimar Johne1
1German Federal Institute for Risk Assessment, Max-Dohrn-Straße 8-10, 10589 Berlin, Germany.
Microorganisms
|March 29, 2023
Summary
Rotavirus A (RVA) was detected at low levels in wild boar, roe deer, and fallow deer in Germany. Specific RVA genotypes were found in different ungulate species, suggesting host associations.
Area of Science:
- Veterinary Virology
- Wildlife Epidemiology
- Zoonotic Disease Surveillance
Background:
- Rotavirus A (RVA) is a significant cause of diarrhea in humans and animals.
- RVA surveillance in wild ungulates remains limited, posing a gap in understanding its epidemiology.
- Investigating RVA in wildlife is crucial for assessing potential zoonotic risks and interspecies transmission.
Purpose of the Study:
- To determine the prevalence and genetic diversity of Rotavirus A in hunted wild ungulates in Brandenburg, Germany.
- To identify specific RVA genotypes circulating within wild boar, roe deer, fallow deer, and red deer populations.
- To explore potential associations between RVA genotypes and specific ungulate host species.
Main Methods:
- Real-time RT-PCR was employed to detect RVA RNA in intestinal contents of hunted wild ungulates.
- RT-PCR products were sequenced for precise genotyping of detected RVA strains.
- Samples from wild boar (n=197), roe deer (n=152), fallow deer (n=95), and red deer (n=28) were analyzed.
Main Results:
- RVA RNA was detected in 1.0% of wild boar, 1.3% of roe deer, and 2.1% of fallow deer; no RVA was found in red deer.
- Genotyping revealed G3P[13] strains in wild boar (related to pig/wild boar strains), G10P[15] in roe deer (related to domestic ruminant strains), and G3P[3] in fallow deer.
- The study indicated a low overall prevalence of RVA in these wild ungulate populations.
Conclusions:
- Rotavirus A circulates at low levels in wild ungulates in Germany, with distinct genotypes identified in different species.
- Preliminary evidence suggests potential host-specific associations for certain RVA genotypes.
- Further research with larger sample sizes is recommended to confirm these genotype-host associations and understand transmission dynamics.
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