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Updated: Aug 5, 2025

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

Published on: September 25, 2016

Clot Imaging Using Photostable Nanodiamond.

Samuel J Francis¹, Marco D Torelli², Nicholas A Nunn²

¹Division of Hematology, Duke University Medical Center, Duke University, Durham, NC 27710, USA.

Nanomaterials (Basel, Switzerland)

|March 29, 2023

Summary

This summary is machine-generated.

Fluorescent nanodiamonds overcome photobleaching limitations in microscopy, enabling better visualization of blood clot formation and lysis. This advancement is crucial for understanding thrombosis and developing new anticoagulant therapies.

Keywords:

blood fluorescence imaging microscopy nanodiamond nanoparticles thrombus

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Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

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Published on: September 25, 2016

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Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

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Published on: March 22, 2020

Area of Science:

Biomedical Engineering
Materials Science
Hematology

Background:

Thrombosis is a major cause of death, yet its mechanisms remain incompletely understood.
Current intravital microscopy techniques for studying thrombus formation are limited by fluorophore photodegradation.
This limits longitudinal studies of clot progression and resolution.

Purpose of the Study:

To evaluate fluorescent nanodiamonds (FNDs) as a photostable alternative to organic fluorophores for labeling blood clots.
To demonstrate the utility of FNDs in visualizing clot formation and lysis in ex vivo models.
To compare FND labeling with traditional organic fluorophores.

Main Methods:

Model ex vivo clots were created using fibrinogen labeled with either FNDs or organic fluorophores.
Intravital fluorescence microscopy was used to image clot formation.
Tissue plasminogen activator (tPa) was added to induce and visualize clot lysis.

Main Results:

FNDs provided comparable morphology to organic fluorophores without photobleaching over time.
FNDs enabled clear visualization of clot formation.
FNDs allowed for the observation of clot lysis in response to tPa.

Conclusions:

Fluorescent nanodiamonds are a viable, photostable alternative for labeling blood clots in microscopy studies.
FNDs facilitate longitudinal imaging of thrombus dynamics, including progression and resolution.
This technology can advance research into deep vein thrombosis (DVT) and pulmonary embolism (PE).