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Single-dye NIR-II chemiluminescence system for H2O2 imaging.
Zong Chang1, Chenchen Liu1,2, Like Guo3
1Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology and Center for Biomedical Optics and Molecular Imaging, Shenzhen Institute of Advanced Technology, CAS, Shenzhen 518055, China. qchao.sun@siat.ac.cn.
Chemical Communications (Cambridge, England)
|March 29, 2023
Summary
Researchers developed a novel single-dye near-infrared-II (NIR-II) chemiluminescence (CL) system for enhanced glucose sensing, tumor therapy, and in vivo imaging. A BASZn nanoenzyme significantly boosted CL intensity, enabling new applications.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Chemiluminescence (CL) imaging in the second near-infrared window (NIR-II) offers deep tissue penetration and reduced background autofluorescence.
- Developing efficient and sensitive NIR-II CL systems remains a challenge for biomedical applications.
- Single-dye systems are desirable for simplicity and cost-effectiveness.
Purpose of the Study:
- To develop a novel, highly efficient single-dye NIR-II chemiluminescence (CL) system.
- To investigate the CL enhancement mechanism using a BASZn nanoenzyme.
- To demonstrate the system's utility in glucose sensing, tumor therapy, and in vivo hydrogen peroxide (H2O2) imaging.
Main Methods:
- Fabrication of biocompatible CL nanoparticles utilizing a single dye.
- Integration of a BASZn nanoenzyme to enhance CL intensity.
- Establishment of a novel electron transfer model through theoretical and experimental analysis.
- Demonstration of applications including glucose sensing, photothermal therapy, and in vivo H2O2 imaging.
Main Results:
- An efficient single-dye NIR-II CL system with emission peaking around 1000 nm was successfully developed.
- A remarkable CL intensity enhancement of approximately three orders of magnitude was achieved with the BASZn nanoenzyme.
- The system demonstrated effective performance in glucose sensing, tumor therapy, and in vivo H2O2 imaging.
- A novel electron transfer model, distinct from the conventional HOMO-LUMO model, was elucidated.
Conclusions:
- The developed single-dye NIR-II CL system offers significant advantages in sensitivity and efficiency.
- The BASZn nanoenzyme plays a crucial role in enhancing CL intensity through a novel electron transfer pathway.
- This work provides valuable insights and guidelines for the rational design of advanced NIR-II CL systems for biomedical applications.
