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Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic
Haobo Sun1,2, Jingliang Liu3, Jinming Kong1
1School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, P. R. China.
ACS Applied Materials & Interfaces
|March 29, 2023
Summary
This study presents a novel biosensor for precise microRNA (miRNA) quantification, crucial for early cancer detection. The advanced system achieves ultra-low detection limits, paving the way for improved lung cancer screening.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Molecular Biology
Background:
- Accurate microRNA (miRNA) quantification is vital for cancer diagnosis but presents significant challenges.
- Existing methods often lack the sensitivity required for early disease detection.
Purpose of the Study:
- To develop a highly sensitive biosensing platform for quantitative miRNA detection.
- To establish a novel signal amplification strategy for improved analytical performance.
Main Methods:
- Development of a logic gate recognition biosensing platform utilizing a "trinity" molecular recognition mode.
- Implementation of a Zn(TCPP) photocatalytic electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization for signal amplification.
- Optimization of experimental conditions for enhanced detection.
Main Results:
- Achieved an ultra-low detection limit of 4.48 aM for miRNAs.
- Demonstrated a broad linear detection range from 0.1 nM to 10 aM.
- Successfully applied the biosensor for tumor marker detection, showing potential for early cancer screening.
Conclusions:
- The developed biosensing platform offers a significant advancement in miRNA quantification sensitivity.
- The novel PET-RAFT polymerization strategy provides a green and effective method for signal amplification in biosensors.
- This technology holds promise for the early screening of lung cancer patients.
