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Updated: Aug 5, 2025
07:59
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
Published on: August 17, 2022
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Cryo-EM structure of the human Sirtuin 6-nucleosome complex.
Un Seng Chio1,2,3, Othman Rechiche1,2, Alysia R Bryll4,5
1Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.
Biorxiv : the Preprint Server for Biology
|March 30, 2023
Summary
Sirtuin 6 (SIRT6) enzyme structure reveals how it targets histone H3 for deacetylation. This provides molecular insights into SIRT6
Area of Science:
- Biochemistry
- Molecular Biology
- Structural Biology
Background:
- Sirtuin 6 (SIRT6) is a key protein deacetylase/deacylase involved in longevity and cancer.
- The molecular basis for SIRT6's preference for nucleosomal substrates is not well understood.
Conclusions:
- The determined structure provides molecular insights into how SIRT6 deacetylates histone H3 at K9 and K56 residues.
- This structural understanding can inform the development of small-molecule modulators targeting SIRT6 for therapeutic purposes.
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