Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: OR7A10 GPCR engineering boosts CAR-NK therapy against solid tumours.

Nature·2026
Same author

Genomic features of clonal hematopoiesis-associated genes in primary and CAR T-cell-related secondary T-cell malignancies.

Blood neoplasia·2026
Same author

Utility of liquid-based cytology and cell block obtained by vitrectomy under infusion to diagnose vitreoretinal lymphoma.

Japanese journal of ophthalmology·2026
Same author

Potential and biases of large language model simulation for public surveys on Alzheimer's disease therapies.

Journal of Alzheimer's disease : JAD·2026
Same author

IgA is necessary and sufficient to prevent norovirus infection in mice.

Science translational medicine·2026
Same author

Large-scale, spatially resolved panoramic CRISPR screening in native tissue environments using Perturb-DBiT.

Nature biotechnology·2026

Related Experiment Video

Updated: Aug 5, 2025

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
11:32

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

Published on: November 13, 2021

10.6K

Cas12a/Cpf1 knock-in mice enable efficient multiplexed immune cell engineering.

Matthew B Dong, Kaiyuan Tang, Xiaoyu Zhou

    Biorxiv : the Preprint Server for Biology
    |March 30, 2023
    PubMed
    Summary

    Researchers developed new transgenic mice using Cas12a technology for advanced CRISPR gene editing. This enables efficient multiplexed gene perturbations in various immune cells, advancing immunological discovery and immune gene engineering.

    More Related Videos

    The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice
    12:04

    The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice

    Published on: November 1, 2015

    17.7K
    Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
    12:04

    Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

    Published on: March 10, 2023

    3.7K

    Related Experiment Videos

    Last Updated: Aug 5, 2025

    Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
    11:32

    Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

    Published on: November 13, 2021

    10.6K
    The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice
    12:04

    The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice

    Published on: November 1, 2015

    17.7K
    Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
    12:04

    Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

    Published on: March 10, 2023

    3.7K

    Area of Science:

    • Molecular Biology
    • Immunology
    • Gene Editing Technologies

    Background:

    • CRISPR-Cas9 systems have revolutionized genetic research but face limitations in simultaneous multiplexed gene editing.
    • The inability of Cas9 to process its own CRISPR RNAs (crRNAs) hinders efficient multiplexed perturbations, particularly with pseudoviral vectors.

    Conclusions:

    • Conditional and constitutive LbCas12a knock-in mice enable efficient multiplexed gene editing in diverse immune cells.
    • This technology expands the capabilities of CRISPR-based gene editing for immunological research and immune gene engineering.
    • The developed toolkit offers broad applicability for fundamental discoveries and therapeutic development.