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Verhoeff van Gieson staining in laser-irradiated gingival tissues
Joseph A Esposito1, Nathan Estrin2, Steve A McClain3
1Department of Biology, School of Science, Siena College, Loudonville, NY, USA.
Journal of Histotechnology
|March 30, 2023
Summary
The Verhoeff van Gieson (VVG) staining method effectively visualizes thermal damage depth in gingival tissues, comparable to hematoxylin & eosin (H&E) staining. VVG staining may offer clearer interpretation for less experienced individuals.
Area of Science:
- Oral Biology
- Biomedical Engineering
- Histopathology
Background:
- Verhoeff van Gieson (VVG) staining is used for thermal tissue effects but rarely for periodontal tissues.
- Hematoxylin & eosin (H&E) is a conventional method for tissue analysis.
Purpose of the Study:
- To compare the quality and effectiveness of VVG staining versus H&E staining.
- To assess their utility in measuring thermal effects in gingival tissues.
Main Methods:
- Bovine mandibular teeth periodontal tissues were treated with surgical lasers (10,600 nm, 970 nm, 445 nm) at 2 W.
- Coagulation zone depth was measured in tissues stained with H&E and VVG.
- Statistical analysis used the Wilcoxon signed-rank test.
Main Results:
- No statistically significant difference was found between H&E and VVG staining methods in measuring thermal effects (P=0.23).
- VVG staining provided better visualization of thermal damage depth.
Conclusions:
- VVG staining is a viable and potentially superior method for assessing laser-induced thermal damage in periodontal tissues.
- VVG staining may simplify the interpretation of thermal penetration depth, even for less experienced personnel.
