Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Generation of Action Potential in Skeletal Muscles01:24

Generation of Action Potential in Skeletal Muscles

4.7K
Every cell in the body maintains a membrane potential due to an uneven distribution of positive and negative charges across its plasma membrane. The membrane potential is measured in millivolts and quantifies the difference in charge across the membrane.
Like neurons, muscle cells are also regarded as excitable due to their capacity to change in response to stimuli, primarily due to voltage-gated ion channels embedded in their plasma membranes, which get activated by alterations in the...
4.7K
Excitation-Contraction Coupling in Skeletal Muscles01:20

Excitation-Contraction Coupling in Skeletal Muscles

8.6K
Excitation-contraction coupling is a series of events that occur between generating an action potential and initiating a muscle contraction. It occurs at the triad, a structure found in skeletal muscle fibers that comprise a T-tubule and terminal cisternae of the sarcoplasmic reticulum on each side. These triads are visible in longitudinally sectioned muscle fibers. They are typically located at the A-I junction — the junction between the A and I bands of the sarcomere.
When an action...
8.6K
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

5.0K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.0K
Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

2.0K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing Maturation of Human Neuromuscular Organoids via Electrical Stimulation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

A spinal circuit for skilled locomotion.

Current biology : CB·2025
Same author

A Validated Regulatory Network for Th17 Cell Specification.

Cell·2025
Same author

The two faces of MyoD: repressor and activator of gene expression during myogenesis.

Genes & development·2025
Same author

Direct specification of lymphatic endothelium from mesenchymal progenitors.

Nature cardiovascular research·2025
Same author

Technologies for profiling the impact of genomic variants on transcription factor binding.

Medizinische Genetik : Mitteilungsblatt des Berufsverbandes Medizinische Genetik e.V·2024
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 4, 2025

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
09:19

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Published on: February 15, 2021

4.9K

Visualizing MyoD Oscillations in Muscle Stem Cells.

Ines Lahmann1,2, Carmen Birchmeier3,4

  • 1Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Developmental Biology/Signal Transduction Group, Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2023
PubMed
Summary
This summary is machine-generated.

Muscle stem cell differentiation is controlled by oscillating MyoD expression, regulated by Hes1. Disrupting these oscillations impairs muscle growth and repair, highlighting their importance in stem cell balance.

Keywords:
BioluminescenceDynamic gene expressionMuscle stem cellMyoDOscillationTime-lapse imaging

More Related Videos

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

4.2K
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
10:45

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Published on: July 26, 2017

10.2K

Related Experiment Videos

Last Updated: Aug 4, 2025

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
09:19

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Published on: February 15, 2021

4.9K
Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

4.2K
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
10:45

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Published on: July 26, 2017

10.2K

Area of Science:

  • Muscle stem cell biology
  • Gene regulation
  • Developmental biology

Background:

  • MyoD is a key transcription factor for muscle differentiation.
  • MyoD expression is known to oscillate in muscle stem cells.
  • The regulation and functional significance of these oscillations are not fully understood.

Purpose of the Study:

  • To investigate the role of Hes1 in regulating MyoD oscillations.
  • To understand how MyoD oscillations influence muscle stem cell behavior.
  • To describe methods for monitoring dynamic MyoD gene expression.

Main Methods:

  • Utilized time-lapse imaging with luciferase reporters.
  • Monitored dynamic MyoD gene expression in myogenic cells.
  • Investigated the effects of Hes1 ablation on MyoD oscillations.

Main Results:

  • MyoD expression oscillates with a period of approximately 3 hours in muscle stem cells.
  • Hes1 acts as a repressor, driving the oscillatory expression of MyoD.
  • Ablation of Hes1 leads to unstable MyoD oscillations and prolonged sustained expression.
  • Impaired MyoD oscillations interfere with muscle stem cell maintenance, growth, and repair.

Conclusions:

  • Oscillations of MyoD and Hes1 are crucial for maintaining the balance between muscle stem cell proliferation and differentiation.
  • Hes1-mediated regulation of MyoD oscillations is essential for proper muscle development and regeneration.
  • Dynamic monitoring of gene expression provides insights into stem cell regulation.