Epitranscriptome Mapping of N⁶-Methyladenosine Using m⁶A Immunoprecipitation with High Throughput Sequencing in...

N⁶-Methyladenosine (m⁶A), one of the most abundant chemical modifications in mRNA (epitranscriptome), contributes to the regulation of biological processes by iterating gene expression post-transcriptionally. A number of publications on m⁶A modification have escalated in the recent past, due to the advancements in profiling m⁶A along the transcriptome using different approaches. The vast majority of studies primarily focused on m⁶A modification on cell lines but not primary cells. We present in this chapter a protocol for m⁶A immunoprecipitation with high throughput sequencing (MeRIP-Seq) that profiles m⁶A on mRNA with merely 100 μg total RNA worth of muscle stem cells as starting material. With this MeRIP-Seq, we observed epitranscriptome landscape in muscle stem cells.