Morphological and chemical profiling for forensic hair examination: A review of quantitative methods

Within the past two decades, there have been many studies for quantitative analysis on human hair samples. Microscopical and chemical analysis techniques have been used to analyze various aspects of hair regarding biological, chemical, anthropological, cosmetic, and forensic applications. Studies have attempted to develop quantification methods to increase the evidentiary value of hair in forensic casework. The literature reviewed in this paper provides some of the current techniques used for forensic examinations and quantitative methods. Although microscopical analysis has been scrutinized in the past, using chemical and microscopical techniques can provide a myriad of information. The extraction of DNA from hair provides high-value evidence; however, it may not be readily available and may yield inconclusive results. Hair analysis can be used for many forensic applications such as comparison, toxicology, and exposure analysis. In this article, we will review published research material regarding chemical and microscopical techniques for human hair analysis. Aspects considered for this review were the sample size requirement for analysis and the destructive nature of the instrumental method. This review will focus on both macro and micro quantitative methods for human hair analysis.