Related Experiment Video

Updated: Aug 4, 2025

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

38.8K

Comparison of four DNA extraction kits efficiency for 16SrDNA microbiota profiling of diverse human samples

Sandrine Le Gall-David1, Gaëlle Boudry1, Sylvie Buffet-Bataillon1,2

  • 1Institut Numecan, INSERM, INRAE, Univ Rennes, Rennes, France.

Future Science OA
|April 3, 2023
PubMed
Abstract

Keywords:
16S rRNA gene sequencingDNA bindingIlluminabacterial DNA extractionbead-beatingdata analysisenzymatic lysishuman microbiotalow biomassnext generation sequencing

More Related Videos

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

28.6K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

19.8K

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
39.6K