Durian Shell-Mediated Simple Green Synthesis of Nanocopper against Plant Pathogenic Fungi

The synthesis of fungicides in eco-friendly and cost-effective ways is significantly essential for agriculture. Plant pathogenic fungi cause many ecological and economic issues worldwide, which must be treated with effective fungicides. Here, this study proposes the biosynthesis of fungicides, which combines copper and Cu₂O nanoparticles (Cu/Cu₂O) synthesized using durian shell (DS) extract as a reducing agent in aqueous media. Sugar and polyphenol compounds contained in DS, as the main phytochemicals acting in the reduction procedure, were extracted under different temperatures and duration conditions to obtain the highest yields. We confirmed the extraction process performed at 70 °C for 60 min to be the most effective in extracting sugar (6.1 g/L) and polyphenols (22.7 mg/L). We determined the suitable conditions for Cu/Cu₂O synthesis using a DS extract as a reducing agent for a synthesis time of 90 min, a volume ratio of DR extract/Cu²⁺ of 15:35, an initial pH solution of 10, a synthesis temperature of 70 °C, and a CuSO₄ concentration of 10 mM. The characterization results of as-prepared Cu/Cu₂O NP showed a highly crystalline structure of Cu₂O and Cu with sizes estimated in the range of 40-25 nm and 25-30 nm, respectively. Through in vitro experiments, the antifungal efficacy of Cu/Cu₂O against Corynespora cassiicola and Neoscytalidium dimidiatum was investigated by the inhibition zone. The green-synthesized Cu/Cu₂O nanocomposites, which are potential antifungals against plant pathogens, exhibited excellent antifungal efficacy against both Corynespora cassiicola (MIC = 0.25 g/L, the diameter of the inhibition zone was 22.00 ± 0.52 mm) and Neoscytalidium dimidiatum (MIC = 0.0625 g/L, the diameter of the inhibition zone was 18.00 ± 0.58 mm). Cu/Cu₂O nanocomosites prepared in this study could be a valuable suggestion for the control of plant pathogenic fungi affecting crop species globally.