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Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
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Evaluation of Bach1 mRNA expression in patients with chronic kidney disease: A preliminary study

Denise Mafra1,2,3, Livia Alvarenga1,2,3, Marcia Ribeiro1

  • 1Graduate Program in Biological Sciences - Physiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro (RJ), Brazil.

Hemodialysis International. International Symposium on Home Hemodialysis
|April 3, 2023
PubMed
Abstract

Insights

Chronic kidney disease (CKD) patients on hemodialysis (HD) show higher Bach1 mRNA expression than those on peritoneal dialysis (PD) or conservative treatment. This suggests Bach1 may play a role in inflammation associated with HD in CKD.

Area of Science:

  • Biochemistry
  • Nephrology
  • Molecular Biology

Background:

  • BTB and CNC homology 1 (Bach1) antagonizes nuclear factor erythroid 2-related factor-2 (Nrf2), inhibiting antioxidant enzyme synthesis and promoting inflammation.
  • Bach1 may be a therapeutic target for managing inflammation in chronic kidney disease (CKD).
  • No clinical studies have investigated Bach1 expression in CKD patients undergoing different treatments.

Purpose of the Study:

  • To evaluate Bach1 mRNA expression in peripheral blood mononuclear cells of CKD patients.
  • To compare Bach1 mRNA levels across different CKD treatment groups: conservative (nondialysis), hemodialysis (HD), and peritoneal dialysis (PD).

Main Methods:

  • Quantitative real-time polymerase chain reaction was used to measure mRNA expression of Bach1, Nrf2, NF-kB, and heme oxygenase 1 (HO-1).
  • Malondialdehyde (MDA) was assessed as a marker of lipid peroxidation.
  • Peripheral blood mononuclear cells were collected from 20 HD patients, 15 PD patients, and 13 nondialysis CKD patients.

Main Results:

  • Bach1 mRNA expression was significantly higher in CKD patients undergoing HD compared to those on PD and nondialysis treatment (p < 0.007).
  • No significant differences were observed in the mRNA expression of HO-1, NF-kB, or Nrf2 among the treatment groups.
  • Dialysis patients generally exhibited higher inflammation levels.

Conclusions:

  • CKD patients on hemodialysis demonstrate an upregulation of Bach1 mRNA expression.
  • The findings suggest a potential role for Bach1 in the inflammatory processes associated with hemodialysis in CKD.
  • Further research is needed to explore the relationship between Nrf2 and Bach1 expression in CKD patients.
Keywords:
BTB and CNC homology 1 (Bach1)NF-kBNrf2chronic kidney diseasedialysisinflammation

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