Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toward standardized iPSC testing: Insights from a multi-year international Quality Assessment Round.

Stem cell reports·2026
Same author

Evaluation of DMSO-free cryopreservation reagent XT-Thrive for establishment of mesenchymal stem cell bank platform.

Frontiers in bioengineering and biotechnology·2026
Same author

Relevance of cerebral microbleeds with leaky blood brain barrier in traumatic brain injury and protective role of interferon-gamma: A cohort study.

Cerebral circulation - cognition and behavior·2026
Same author

Dynamic modulation of ionic and electronic pathways in flexible SnS<sub>2</sub>-based interdigitated solid-state supercapacitors.

Nanoscale·2026
Same author

Epidemiology of Fencing Injuries Presenting to Emergency Departments in the United States: 2003 to 2022.

Clinical journal of sport medicine : official journal of the Canadian Academy of Sport Medicine·2025
Same author

Scalable, High-Density Expansion of Human Mesenchymal Stem Cells on Microcarriers Using the Bach Impeller in Stirred-Tank Reactors.

Biotechnology and bioengineering·2025

Related Experiment Video

Updated: Aug 4, 2025

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
09:03

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Published on: March 17, 2023

1.9K

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow.

Alan Tin Lun Lam1, Premkumar Jayaraman2, Abigail Becker2

  • 1Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A STAR); Alan_Lam@bti.a-star.edu.sg.

Journal of Visualized Experiments : Jove
|April 3, 2023
PubMed
Summary

This study presents a closed, semi-automated process for harvesting human mesenchymal stem cells (hMSCs) using counterflow centrifugation. This method ensures high cell recovery and viability, addressing key manufacturing challenges for cell therapies.

More Related Videos

Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
10:30

Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

Published on: December 8, 2016

10.1K
Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells
13:44

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Published on: November 10, 2023

2.0K

Related Experiment Videos

Last Updated: Aug 4, 2025

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
09:03

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Published on: March 17, 2023

1.9K
Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
10:30

Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

Published on: December 8, 2016

10.1K
Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells
13:44

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Published on: November 10, 2023

2.0K

Area of Science:

  • Cell therapy manufacturing
  • Bioprocessing engineering
  • Stem cell biology

Background:

  • Human mesenchymal stem cells (hMSCs) show therapeutic potential, but manufacturing bottlenecks hinder clinical translation.
  • Scaling up hMSC production requires addressing issues like reproducibility, cost, and regulatory compliance.
  • Closed and automated systems are crucial for efficient and consistent cell therapy manufacturing.

Purpose of the Study:

  • To develop a closed, semi-automated process for passaging and harvesting Wharton's jelly-derived hMSCs (WJ-hMSCs).
  • To evaluate the efficiency and quality of WJ-hMSCs processed using counterflow centrifugation.
  • To demonstrate the applicability of the developed protocol for various cell types and scales.

Main Methods:

  • Expansion of WJ-hMSCs in regulatory-compliant serum-free xeno-free (SFM XF) medium.
  • Development of a closed, semi-automated harvesting protocol utilizing counterflow centrifugation for cell concentration and washing.
  • Assessment of cell proliferation, morphology, surface marker expression, CFU-F, trilineage differentiation, and cytokine profiles.

Main Results:

  • Comparable cell proliferation and morphology were observed between SFM XF and traditional serum-containing media.
  • The semi-automated harvesting protocol achieved high cell recovery (~98%) and viability (~99%).
  • Counterflow centrifugation maintained critical WJ-hMSC quality attributes, including surface markers, differentiation potential, and functional profiles.

Conclusions:

  • A closed, semi-automated process using counterflow centrifugation effectively addresses WJ-hMSC manufacturing challenges.
  • The developed protocol ensures high cell quality and yield, facilitating scalable cell therapy production.
  • This adaptable harvesting system can be applied to various adherent and suspension cells for small- to medium-scale processing.