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Drug interference with urine protein determination.

S Yosselson-Superstine, Y Sinai

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |January 1, 1986
    PubMed
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    This study investigated drug interference in urine protein tests. Gentamicin was the only drug found to interfere with the Ponceau S dye test for urine protein estimation.

    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Pharmacology

    Background:

    • Accurate urine protein measurement is crucial for diagnosing and monitoring various kidney diseases.
    • Several medications are known to potentially interfere with common urine protein assays, leading to inaccurate results.
    • This study evaluated potential drug interferences across multiple urine protein estimation methods.

    Observation:

    • Five urine protein determination techniques were compared: Albustix, heat/acetic acid turbidity, sulphosalicylic acid, trichloroacetic acid, and Ponceau S dye test (Urin-Pak).
    • Patients received therapeutic doses of common drugs including penicillin G, ampicillin, cefoxitin, gentamicin, and others.
    • Albumin was added in vitro to urine samples to simulate varying protein concentrations.

    Findings:

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    • The semi-quantitative Albustix and heat/acetic acid tests showed no significant drug interference.
    • The quantitative sulphosalicylic acid and trichloroacetic acid tests were also unaffected by the tested drugs.
    • Gentamicin demonstrated significant interference exclusively with the Ponceau S dye-protein complex test (Urin-Pak).

    Implications:

    • Clinicians should be aware of potential interferences when interpreting urine protein results, particularly when using the Ponceau S dye method.
    • The findings suggest that gentamicin does not interfere with most common urine protein assays, except for the Ponceau S dye test.
    • Further research may be warranted to confirm these findings and explore the mechanism of gentamicin interference with the Ponceau S dye test.