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CRISPR/Cas12a-enhanced single-molecule counting for sensitive detection of flap endonuclease 1 activity at the
Ning-Ning Zhao1, Xiaorui Tian1, Fei Ma2
1College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn.
Abstract:
We develop a new fluorescent biosensor for flap endonuclease 1 (FEN1) assay based on CRISPR/Cas12-enhanced single-molecule counting. This biosensor is simple, selective, and sensitive with a detection limit of 2.325 × 10-5 U and it is applicable for inhibitor screening, kinetic parameter analysis, and quantifying cellular FEN1 with single-cell sensitivity.
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