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Updated: Aug 4, 2025

Problem-Solving Before Instruction PS-I: A Protocol for Assessment and Intervention in Students with Different Abilities
Published on: September 11, 2021
Imagining, designing, and interpreting experiments: Using quantitative assessment to improve instruction in
Aaron B Coleman1, Kyla Lorenzo1, Flannery McLamb1
1School of Biological Sciences, University of California, San Diego, California, 92093, USA.
This study developed an assessment to evaluate undergraduate scientific reasoning skills in cellular and molecular biology. The assessment identified common errors and measured significant improvements in student abilities after instruction.
Area of Science:
- Biology Education
- Scientific Reasoning Assessment
- Cellular and Molecular Biology
Background:
- Effective science education requires understanding student challenges in developing scientific reasoning skills.
- Undergraduate students often struggle with core scientific reasoning competencies such as hypothesis formation, experimental design, and data interpretation.
- Identifying specific reasoning errors is crucial for improving pedagogical approaches.
Purpose of the Study:
- To design and validate an assessment tool measuring undergraduate scientific reasoning in cellular and molecular biology.
- To identify common errors in hypothesis formation, experimental design, and data interpretation.
- To evaluate the effectiveness of the assessment in tracking improvements in scientific reasoning across different instructional levels.
Main Methods:
- Development of an assessment with intermediate-constraint, free-response questions and a defined rubric.
- Application of the assessment to undergraduate students in a senior-level biochemistry laboratory course and a first-year introductory biology lab course.
- Statistical analysis to measure improvements in scientific reasoning and identify common errors.
Main Results:
- The assessment demonstrated statistically significant improvements in scientific reasoning among senior-level biochemistry students.
- A greater improvement was observed in biochemistry lab students compared to first-year introductory biology students.
- Common errors included hypotheses restating observations and inappropriate comparisons to absent experimental controls, particularly among introductory students.
Conclusions:
- The developed assessment is a valuable tool for measuring scientific reasoning improvement in undergraduate biology.
- Targeting specific errors, such as hypothesis formulation and the use of experimental controls, can enhance science instruction.
- Difficulties with experimental controls appear widespread among undergraduate students, necessitating further pedagogical focus.
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