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Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip
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Integration of Extracellular Matrices into Organ-on-Chip Systems.

Hazal Kutluk1,2, Effie E Bastounis3,4, Iordania Constantinou1,2

  • 1Institute of Microtechnology (IMT), Technical University of Braunschweig, Alte Salzdahlumer Str. 203, 38124, Braunschweig, Germany.

Advanced Healthcare Materials
|April 5, 2023
PubMed
Summary
This summary is machine-generated.

Integrating extracellular matrix (ECM) into Organ-on-Chip (OoC) platforms is crucial for biomimicry. This review explores current methods for designing and assessing ECMs in OoCs, addressing challenges in reproducibility and characterization for advanced mechanobiology applications.

Keywords:
PDMScell culturesextracellular matrixhydrogelsmechanobiologyorgans-on-chip

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • The extracellular matrix (ECM) provides structural support and regulates cell behavior.
  • Organ-on-Chip (OoC) platforms aim to replicate organ functions in vitro.
  • Integrating functional ECMs into OoCs is essential for improving biomimicry but faces significant challenges.

Purpose of the Study:

  • To review the current state-of-the-art in designing and assessing in vitro ECM environments for OoC applications.
  • To discuss the challenges and limitations in mimicking native ECM properties within OoC platforms.
  • To highlight the importance of ECM characterization and material selection for OoC development.

Main Methods:

  • Review of synthetic and natural hydrogels used in OoC platforms.
  • Analysis of polydimethylsiloxane (PDMS) applications as substrates, coatings, or membranes.
  • Discussion of methods for characterizing ECM properties in vitro and within OoCs.

Main Results:

  • Various hydrogels and PDMS-based materials show potential for mimicking native ECM.
  • Current methods for modulating and assessing ECM properties on OoCs are underdeveloped.
  • The interplay between materials, OoC architecture, and ECM characterization complicates research.

Conclusions:

  • Improving biomimetic ECM integration in OoCs can enhance their use as alternatives to animal models.
  • Tailored ECM properties are key to advancing OoCs for mechanobiology studies.
  • Standardized methods for ECM design and assessment are needed for OoC research reproducibility.