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CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Updated: Aug 4, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

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CRISPR-Based Biosensing Strategies: Technical Development and Application Prospects.

Tian Tian¹, Xiaoming Zhou¹

¹School of Life Sciences, South China Normal University, Guangzhou, China;

Annual Review of Analytical Chemistry (Palo Alto, Calif.)

|April 5, 2023

Summary

CRISPR-Cas biosensing offers innovative strategies for detecting nucleic acids and non-nucleic acids. This review details CRISPR bioassay properties, technical advancements, and commercialization challenges for next-generation diagnostics.

Keywords:

CRISPR biosensing nucleic acids proteins small molecules

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Area of Science:

Biotechnology
Molecular Biology
Biosensing

Background:

CRISPR-Cas systems are rapidly evolving for biosensing applications.
These systems offer unique properties for novel detection strategies.
Existing CRISPR platforms enable detection of both nucleic and non-nucleic acids.

Purpose of the Study:

To review the core biochemical properties of CRISPR bioassays.
To highlight technical developments for enhanced CRISPR detection.
To analyze commercialization obstacles and future opportunities for CRISPR technology.

Main Methods:

Review of CRISPR-Cas system biochemical properties.
Analysis of technical advancements in CRISPR biosensing.
Discussion of commercialization challenges and future directions.

Main Results:

CRISPR bioassays leverage programmability, efficiency, and specificity.
Improvements focus on sensitivity, quantification, multiplexing, and one-pot assays.
Advanced sensors and expanded applications are emerging.

Conclusions:

CRISPR-Cas biosensing is a promising field with significant potential.
Overcoming commercialization hurdles is key to widespread adoption.
Future research should focus on refining detection capabilities and applications.