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Particulate Standard Establishment for Absolute Quantification of Nanoparticles by LA-ICP-MS.

Wenhe Luo1,2, Fengliang Dong3, Meng Wang1,2

  • 1CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, CAS-HKU Joint Laboratory of Metallomics on Health & Environment, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.

Analytical Chemistry
|April 5, 2023
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Summary

This study introduces a novel method for precisely quantifying nanoparticles (NPs) using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The approach establishes accurate calibration standards, enabling reliable single-NP detection and quantification for various applications.

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Area of Science:

  • Nanotechnology
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Nanoparticle (NP) detection is crucial in single-molecule analysis, demanding high sensitivity and resolution.
  • Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is used for NP tracking but faces challenges in quantitative calibration due to a lack of standards and matrix effects.

Purpose of the Study:

  • To develop a robust method for the absolute quantification of nanoparticles using LA-ICP-MS.
  • To overcome the limitations of current quantitative calibration methods for NP analysis.

Main Methods:

  • Precise synthesis and nanoscale characterization of gold nanoparticles (AuNPs) to create quantitative standards.
  • On-demand NP distribution and deep learning-assisted NP counting for accurate calibration.
  • Development of an LA-ICP-MS method utilizing these standards for precise NP quantification.

Main Results:

  • Established gold NP standards covering mass ranges from sub-femtogram to picogram levels with high accuracy and precision.
  • Demonstrated an unambiguous relationship between sampled NP number and mass spectral signal.
  • Enabled the study of factors influencing sample capture and signal transduction in LA-ICP-MS analysis.
  • Developed an LA-ICP-MS method with single-NP sensitivity and single-cell quantification capability.

Conclusions:

  • The developed strategy provides a reliable method for absolute nanoparticle quantification.
  • This breakthrough facilitates the investigation of toxicological and diagnostic issues related to nanoparticle analysis.
  • The approach paves the way for advancements in single-NP detection and quantification using LA-ICP-MS.