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Updated: Aug 4, 2025
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Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
Published on: February 15, 2019
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Total Transit Time and Probiotic Persistence in Healthy Adults: A Pilot Study
Annie Tremblay1, Jeremie Auger1, Zainab Alyousif2
1Rosell Institute for Microbiome and Probiotics, Montreal, Quebec, Canada.
Journal of Neurogastroenterology and Motility
|April 5, 2023
Summary
Probiotic persistence in stool varies by individual gut transit time and microbiota composition. These host factors influence probiotic detection duration after cessation, impacting study design.
Area of Science:
- Gastroenterology
- Microbiome research
- Nutritional science
Background:
- Probiotic efficacy is influenced by gut transit and microbiota.
- Little is known about probiotic persistence after intake stops.
Purpose of the Study:
- Characterize probiotic fecal detection parameters (onset, persistence, duration).
- Examine relationships between probiotic persistence, whole gut transit time (WGTT), and fecal microbiota composition.
Main Methods:
- Pilot, open-label study with 30 healthy adults.
- 2-week probiotic intake followed by 4-week washout.
- Stool collections and WGTT measurement using radio-opaque markers.
Main Results:
- Probiotics detected in feces 1-2 days post-intake; persistence varied.
- Identified 3 WGTT subgroups (Fast, Intermediate, Slow) classified by machine learning.
- One probiotic strain (R0175) showed significantly longer persistence in the intermediate WGTT subgroup.
Conclusions:
- Host factors like WGTT and microbiota composition are crucial for probiotic study design.
- Consideration of these parameters can optimize washout periods, enrollment criteria, and supplementation regimens.
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