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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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A comprehensive benchmarking of differential splicing tools for RNA-seq analysis at the event level

Minghao Jiang1, Shiyan Zhang1, Hongxin Yin1

  • 1Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine, Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 197 Ruijin Er Road, Shanghai 200025, China.

Briefings in Bioinformatics
|April 6, 2023
PubMed
Summary

This study benchmarks 21 RNA alternative splicing tools using simulated data, finding significant discrepancies. SUPPA, DARTS, rMATS, and LeafCutter show superior performance for differential splicing detection.

Keywords:
RNA alternative splicingRNA-seqbenchmarking studynovel splicing events

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