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Updated: Aug 4, 2025
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
Published on: July 18, 2019
Single-Cell Sequencing Data Analysis Unveiled HDAC1 as the Therapeutic Target for Chronic Pancreatitis
1Gastroenterology Department, The First Affiliated Hospital of Suzhou University, 899 Pinghai Road, Gusu District, Suzhou, 215000, Jiangsu, China.
Abstract:
Chronic pancreatitis (CP) as a progressive inflammatory disorder, remains untreatable. The novel treatment strategy for CP is imperative. We attempted to explore the therapeutic biomarkers for CP. The single-cell sequencing data were retrieved from Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) in idiopathic CP were identified, followed by function and pathway annotation, and PPI network established. DEGs of interest were verified in human tissue samples. The function of candidate biomarker was determined in the murine model with CP. A total of 208 genes were specially differentially expressed in idiopathic patients. Functional enrichment analysis showed DEGs were mainly enriched in glycogen catabolic process, RNA splicing, and glucagon signaling pathway. A PPI network centered on HDAC1 was constructed. HDAC1 was overexpressed in CP patients. The murine model with CP was induced by repetitive cerulein treatment. Silencing sh-HDAC1 treatment reversed cerulein-induced inflammatory cells accumulation, high expression of TGF-β1, and collagen 1 in pancreas in vivo. HDAC1 might be served as potential biomarker for CP. The present study provided insights into the molecular mechanism of CP that may be useful in further investigations.
Insights
Researchers identified HDAC1 as a potential therapeutic biomarker for chronic pancreatitis (CP), an untreatable inflammatory disorder. Silencing HDAC1 in a mouse model reversed key disease markers, offering new avenues for CP treatment strategies.
Area of Science:
- Gastroenterology
- Molecular Biology
- Biomarker Discovery
Background:
- Chronic pancreatitis (CP) is a progressive inflammatory disease with no current cure.
- Novel therapeutic strategies and biomarkers are urgently needed for effective CP management.
Purpose of the Study:
- To identify potential therapeutic biomarkers for chronic pancreatitis (CP).
- To investigate the role of identified biomarkers in the molecular mechanisms of CP.
Main Methods:
- Single-cell sequencing data from the Gene Expression Omnibus (GEO) database were analyzed to identify differentially expressed genes (DEGs) in idiopathic CP.
- Functional enrichment analysis and protein-protein interaction (PPI) network construction were performed.
- The expression and function of candidate biomarkers were validated in human tissue samples and a murine model of CP.
Main Results:
- A total of 208 DEGs were identified in idiopathic CP patients, enriched in pathways like glycogen catabolic process and RNA splicing.
- Histone deacetylase 1 (HDAC1) was identified as a key gene in the PPI network and was found to be overexpressed in CP patients.
- Silencing HDAC1 in a mouse model of CP reduced inflammation, TGF-β1 expression, and collagen deposition in the pancreas.
Conclusions:
- HDAC1 is overexpressed in chronic pancreatitis and plays a significant role in disease progression.
- HDAC1 represents a promising therapeutic biomarker for chronic pancreatitis.
- This study provides valuable insights into the molecular pathogenesis of CP, potentially guiding future therapeutic interventions.
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