Related Experiment Video
Updated: Aug 4, 2025

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses
Published on: July 7, 2017
Correlation Between IL-8 Gene Polymorphisms and Pathogenesis of Crohn's Disease
Context:
The risk of inflammatory bowel disease (IBD) is substantially heightened if patients' first-degree relatives have it. The genetic and immune factors related to the disease have attracted great attention, including patients innate genetic polymorphisms. Interleukin-8 (IL-8) plays a vital role in digestive-system diseases, especially in gastrointestinal diseases.
Objective:
The study intended to explore the expression of interleukin-8 (IL-8) in the colon tissues of patients with Crohn's disease and the correlation between its polymorphisms and the disease's occurrence.
Design:
The research team performed a prospective study.
Setting:
The study took place in the Department of Gastroenterology at Zhuji People's Hospital of Zhejiang Province in Zhuji, China.
Participants:
Participants were 100 patients with Crohn's disease at the hospital between November 2016 and June 2018 and 100 healthy individuals. The research team assigned participants with Crohn's disease to the Crohn's disease group and the healthy participants to the control group.
Outcome Measures:
The research team: (1) determined differences in the protein expression of the IL-8 between the groups; (2) examined the conformity of the data to that of the Hardy-Weinberg equilibrium; (3) analyzed the differences in the genotypes and alleles for the IL-8 single nucleotide polymorphisms (SNPs) rs102039, rs103284 and rs105432 between the groups; and (4) for the Crohn's disease group, examined the differences in the disease's location and behavior for the participants with different genotypes.
Results:
The protein expression level of IL-8 in the colon tissues in Crohn's disease group was significantly higher than that in control group (P < .05). The genetic association analysis showed significant correlations between the polymorphisms rs103284 and rs105432 and alleles of the IL-8 gene and the occurrence of Crohn's disease (P < .05), but no associations existed between the gene polymorphism rs102039 and alleles and Crohn's disease (P > .05). Significant correlations existed between the IL-8 gene polymorphisms rs103284 and rs105432 and the disease's location and behavior (P < .05).
Conclusions:
IL-8 had a significantly increased expression in the colon tissues of the participants with Crohn's disease, and some genotypes and alleles for the gene polymorphisms rs103284 and rs105432 were significantly higher in the Crohn's disease group than in the control group. In addition, the disease's location and behavior were significantly different for participants in the Crohn's disease group with different genotypes.
More Related Videos
05:53Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
10:21Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
Published on: September 20, 2024
Related Concept Videos
Inflammatory Bowel Disease II: Crohn's Disease
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF
Cystic Fibrosis: Pathogenesis
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
Single Nucleotide Polymorphisms-SNPs
Inflammatory Bowel Disease I: Ulcerative Colitis
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...