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Next Generation Sequencing for miRNA Detection on the Exhaled Breath Condensate: A Pilot Study
Roberto Cherchi1, Roberto Cusano2, Sandro Orrù3,4
1Operative Unit of Thoracic Surgery, Hospital G. Brotzu, Cagliari, Italy.
Epigenetics Insights
|April 7, 2023
Summary
Exhaled breath condensate (EBC) sampling is a viable, non-invasive method for detecting microRNA (miRNA) profiles. The HiSeq sequencing platform significantly enhances miRNA detection in EBC compared to plasma.
Area of Science:
- Biomarker Discovery
- Molecular Diagnostics
- Environmental Health
Background:
- Exhaled breath condensate (EBC) offers a non-invasive method for detecting biological macromolecules like miRNA.
- Investigating EBC as a potential biomarker for early effects of asbestos exposure is crucial.
Purpose of the Study:
- To assess the feasibility of using EBC as a biomarker for early asbestos exposure effects.
- To compare miRNA profiles in EBC and plasma using different sequencing platforms.
Main Methods:
- Six retired male volunteers participated in the study.
- RNA was extracted from EBC and plasma samples.
- Two sequencing platforms, Illumina MiSeq® and HiSeq®, were utilized.
Main Results:
- The HiSeq® platform identified twice as many unique miRNA as the MiSeq® platform.
- Significant correlations between EBC and plasma miRNA counts were observed (Spearman's correlation).
- Bland-Altman plots indicated improved EBC miRNA detection with the HiSeq® platform.
Conclusions:
- EBC sampling is a suitable, non-invasive method for detecting miRNA profiles in healthy subjects.
- The HiSeq® sequencing platform enhances miRNA detection in EBC.
