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Target response controlled enzyme activity switch for multimodal biosensing detection.

Lu Zhang¹, Haiping Wu¹, Yirong Chen¹

¹Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China.

Journal of Nanobiotechnology

|April 8, 2023

Summary

This summary is machine-generated.

Researchers developed a novel Controllable Enzyme Activity Switch (CEAS) using hemin aggregation. This breakthrough allows for precise, stimulus-responsive enzyme regulation and opens new avenues for advanced biosensing applications.

Keywords:

Controlled enzyme activity Dimerization inactivation G4-Hemin DNAzyme Hemin Multimodal detection

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Area of Science:

Biochemistry
Nanotechnology
Molecular Biology

Background:

Enzyme catalysis research aims to precisely regulate enzyme activity for advanced applications.
Conventional enzymes and nano-enzymes face limitations in stimulus-responsive activity modulation due to structural constraints.

Purpose of the Study:

To develop a novel Controllable Enzyme Activity Switch (CEAS) based on hemin aggregation regulation.
To explore the regulatory mechanism of CEAS and develop multimodal biosensing applications.

Main Methods:

CEAS utilizes the dimerizable inactivation of the catalytically active center hemin.
A DNA template guides G4-Hemin DNAzyme and DNA-Hemin binding to inhibit catalytic activity.
Customizable guide templates enable target-regulated dissociation of hemin dimers, restoring synergistic catalysis.

Main Results:

Demonstrated stimulus-responsive enzyme activity modulation through controlled hemin aggregation and dissociation.
Developed visual and fluorescent protein detection systems with excellent bioanalytical performance by coupling CEAS with DNA recognition techniques.
Achieved target-regulated enzymatic activity switching, overcoming limitations of conventional enzyme regulation.

Conclusions:

The CEAS provides a new paradigm for controllable enzyme activities, enabling customizable enzymatic reaction systems.
This approach overcomes the limitations of conventional enzymes lacking targetable regulation.
CEAS is poised to advance enzyme catalysis and biosensing technologies.