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Updated: Aug 3, 2025

$Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography$

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

A simple goniometer-compatible flow cell for serial synchrotron X-ray crystallography.

Swagatha Ghosh¹, Doris Zorić¹, Peter Dahl¹

¹Department of Chemistry and Molecular Biology, University of Gothenburg, Medicinaregatan 9C, 40530 Gothenburg, Sweden.

Journal of Applied Crystallography

|April 10, 2023

Summary

This summary is machine-generated.

A new, simple flow cell enables serial synchrotron X-ray crystallography (SSX) studies of enzyme reactions using microcrystals. This disposable device facilitates easier structural analysis of biological processes, lowering barriers for researchers new to SSX.

Keywords:

cytochrome c oxidase goniometer-compatible flow cells macromolecular crystallography serial synchrotron X-ray crystallography

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Published on: September 23, 2020

Area of Science:

Structural Biology
Biochemistry
Crystallography

Background:

Serial femtosecond crystallography (SFX) was developed for macromolecular studies at X-ray free electron lasers.
Time-resolved serial crystallography enables investigation of enzyme catalytic mechanisms by studying structural changes during reactions.
Serial synchrotron X-ray crystallography (SSX) extends these methods to synchrotron sources, broadening accessibility.

Purpose of the Study:

To present a simple, disposable flow cell for microcrystal delivery in serial synchrotron X-ray crystallography (SSX).
To demonstrate the utility of this flow cell for time-resolved structural studies of enzyme catalysis.

Main Methods:

Development and implementation of a novel flow cell system using a glass capillary, 3D-printed support, and syringe pump.
Collection of SSX data from microcrystals of *Thermus thermophilus* cytochrome *c* oxidase at MAX IV Laboratory.
Determination of the X-ray structure of cytochrome *c* oxidase using the collected SSX data.

Main Results:

The flow cell is easily mounted, aligned, and disposable, allowing for rapid replacement when blocked.
Successful collection of SSX data from microcrystals of cytochrome *c* oxidase.
Determination of the X-ray structure of cytochrome *c* oxidase to 2.12 Å resolution.

Conclusions:

The developed flow cell provides a simple and effective platform for SSX experiments.
This system may reduce the technical barriers for researchers utilizing SSX methods.
Facilitates structural studies of biological reactions and enzyme mechanisms.