Two-dimensional glass/p-ATP/Ag NPs as multifunctional SERS substrates for label-free quantification of uric acid in sweat
![Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F61682.jpg&w=3840&q=50)
Dechan Lu1, Rongyuan Cai2, Yuqin Liao3
1College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, Fujian 350007, China; College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, Fujian 350007, China.
Abstract:
Abnormal uric acid (UA) content in body fluids can fully reflect the status of metabolism and immunity in the body. We have developed a simple, efficient and label-free surface enhanced Raman scattering (SERS) method for UA detection. Briefly, p-aminothiophenol (p-ATP) was used as the internal standard molecule and linking molecule to prepare a glass/p-ATP/Ag NPs SERS substrate. The Raman characteristic peak of p-ATP at 1076 cm-1 can be used as an internal standard molecule to correct the signal fluctuation of UA detection. The results show that the SERS method owns a linear response with a ranging from 5 × 10-6 to 10-3 M of UA characteristic peak of both 693 cm-1 and 493 cm-1 with a determination coefficient (R2) of 0.9878 and 0.9649, respectively. Additionally, the SERS sensor has been further used for the analysis of UA in sweat and good recoveries were obtained for the sensing of sweat. We believe that the developed SERS substrate has potential for applications in healthcare monitoring.
