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Updated: Aug 3, 2025
Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
Simultaneous observation of the spatial and temporal dynamics of single enzymatic catalysis using a solid-state
Ru-Jia Yu1,2,3, Qiao Li3, Shao-Chuang Liu1,3
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China. yurujia@nju.edu.cn.
Abstract:
We developed a bipolar SiNx nanopore for the observation of single-molecule heterogeneous enzymatic dynamics. Single glucose oxidase was immobilized inside the nanopore and its electrocatalytic behaviour was real-time monitored via continuous recording of ionic flux amplification. The temporal heterogeneity in enzymatic properties and its spatial dynamic orientations were observed simultaneously, and these two properties were found to be closely correlated. We anticipate that this method offers new perspectives on the correlation of protein structure and function at the single-molecule level.
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